Part:BBa_K4205111
lldR
The lldPRD operon(formerly named lct)of Escherichia coli is responsible for aerobic l-lactate metabolism. It includes three genes that form a single transnational unit inducible by growth in l-lactate. The lldD gene encodes the dehydrogenase,lldP encodes the permease,and lldR encodes a regulatory protein. In the absence of l-lactate,probably leading to DNA looping and the repression of transcription. Binding of l-lactate to LldR promotes a conformational change that may disrupt the DNA loop ,allowing the formation of the transcription open complex.[1] The design of the library was made using promoters P9, apFAB54, apFAB303, P11;and the J23104 promoter from the Anderson promoter library .The RBS library design was derived from the Anderson family.The modified L-lactate sensor are highly sensitive and specific.As the exposure lactate concentration increased, reporter gene expression increased accordingly.[2]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 246
- 1000COMPATIBLE WITH RFC[1000]
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